FAQs: MolecuLab 217/218

Q: Why must I rehydrate the DNA samples I received?

DNA samples are dehydrated for stability during long-term storage.  You can give your students extra practice with pipetting by having them rehydrate the samples shortly (within 1 week) before their use.  Alternatively, you can save your precious class time by rehydrating the samples yourself or by having a teaching assistant/student worker rehydrate the samples for you.

Q: What are the band sizes of the DNA Ladder?

The DNA ladder is a 100bp ladder.  The largest band is 2072bp.  The second largest band is 1500bp and each band thereafter is 100bp smaller.

Q: Do the band patterns reflect heterozygous and homozygous conditions?

The specific form of porphyria being tested for is PCT, a heterozygous condition.  Thus, the band patterns revealed by electrophoresis are RFLP patterns that are testing for the presence of particular mutations within the UROD gene.  A band pattern that matches the pattern of the Proband (diagnosed individual) within the same group suggests that that individual has the same mutations as the Proband and that a PCT diagnosis is likely.  The patterns should not be analyzed for and are not meant to reflect homozygous and heterozygous conditions.

Q: How many microliters of 1X loading dye do I need to add to my dried down DNA samples in MolecuLabs 217/218?

Add 12µL of 1X loading dye to rehydrate each sample.

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